Fig. 3

CMH-induced endothelial proliferation occurs predominantly in capillaries and venules. Frozen brain sections taken from mice exposed to 4 days hypoxia (8% O₂) were dual-labelled for CD31 (AlexaFluor-488) and Ki67 (Cy3) (A, B), laminin-111 (AlexaFluor-488) and Ki67 (Cy3) (C, D), or α-SMA (Cy3) and Ki67 (AlexaFluor-488) (E). Scale bars = 100 μm. F Quantification of the number of proliferating endothelial cells (CD31⁺/Ki67⁺ cells)/FOV after 4 days hypoxia. G Time-course of the % of total vessels that are angiogenic. Results are expressed as the mean ± SEM (n = 4 mice/group). Note that the majority (approximately two-thirds) of proliferating endothelial cells were located in capillaries (laminin-111 negative vessels), and one-third in venules, with very few in arterioles