Fig. 2

Leaky vessels are predominantly capillaries. Frozen brain sections taken from mice exposed to 4 days hypoxia (8% O₂) were dual-labelled for CD31 (AlexaFluor-488) and fibrinogen (Cy3) (A, B), or triple-labelled for laminin-111 (AlexaFluor-488), α-SMA (Cy3), and fibrinogen (Cy5) (C). Scale bars = 100 μm. D Quantification of the number of capillaries (laminin-111-negative), venules (laminin-111⁺/α-SMA-negative) or arterioles (α-SMA⁺)/FOV that showed extravascular fibrinogen leak after 4 days hypoxia. E Time-course of the % of vessels showing vascular leak. All results are expressed as the mean ± SEM (n = 4 mice/group). Note that CMH-induced vascular leak occurs predominantly in capillaries (laminin-111 negative), rarely in venules, but never in arterioles