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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Tachycardia and hypertension enhance tracer efflux from the spinal cord

Fig. 5

Hypertension and tachycardia have greater effect on tracer efflux from the spinal white matter than changes in respiration. Quantification of ovalbumin (AFO-647) tracer fluorescence within microscopic whole axial sections, grey matter and white matter. a, b. After injection of AFO-647 into the spinal white matter, the fluorescence intensity of redistributed tracer was measured within the grey and white matter of levels remote from the injection site (C2–6 and T2–T4). Blood vessels were labelled with an endothelial marker (RECA-1), the (*) depicts the injection site in the lateral white matter. c, f, i There was no difference in tracer intensity within the whole axial section or spinal white matter in spontaneous breathing (SB) rats compared with mechanically ventilated controls (MV), although significantly higher signal was detected in the grey matter. d, g, j There was significantly higher fluorescence in hypertensive (High MAP) rats (compared with Low MAP controls) after injection of tracer into the white matter. This was observed within both the grey and white matter. On post hoc analysis significant difference was reached at T2 in the grey matter, as well as at T3 in the white matter and within the whole axial section. e, h, k There were similar findings in tachycardic (High HR) rats compared with controls (Low HR), with higher tracer intensity in tachycardic rats in all parts of the spinal cord. On post hoc analysis, the difference in fluorescence intensities reached significance at T2 except in the white matter. Two-way analysis of variance (ANOVA) post hoc Bonferroni’s, *p < 0.05. All error bars are expressed as ± SEM, n = 10 rats

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