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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Characterization of the blood–brain barrier in genetically diverse laboratory mouse strains

Fig. 4

RNA sequencing of cortical and hippocampal endothelial cells of different inbred mouse strains. Brain endothelial cells for RNA sequencing were isolated from dissected hippocampus and cortex after mechanical and enzymatical dissociation. Age of mice: 2 months. Isolation of EC using anti-CD31 Ab labelling and flow cytometry (A). Pseudocolor flow cytometry dotplots showing the gating strategy to sort for CD31+ endothelial cells. Gating included singlet gating, live cell gating (7AADneg), exclusion of immune cells (CD11b+, CD45+) and inclusion of CD31+ endothelial cells (B). Principal component analysis of normalized RNAseq counts of the deregulated genes (C). Hierarchical clustering heatmap showing z-scores of normalized read counts for the 1000 most variable genes across samples (D)

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