Fig. 3

Vessel density, pericyte coverage and permeability index to NaF of 12 month old mice. Immunofluorescence staining of CD13 (in red, pericyte marker) and vascular basement membrane (Col-IV, in cyan) in the cortex (A) and hippocampus (B). Quantification of vessel density (C) and pericyte coverage (D) in the cortex and hippocampus. N = 2. PI of cortex or hippocampus (E) and ratio of PI hippocampus to PI cortex (F) of different mouse strains of 12 months old animals. N = 3–9. Pink dotted line: PI ratio = 1. Each dot indicates one mouse (C–F). Age of mice: 12 months. Female and male mice were used for analysis. Statistical testing performed using ANOVA. Data are presented as mean ± SD. Scale bars: 100 µm