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Table 1 TEER values and paracellular mannitol permeability (basolateral to apical) in nm/s and percentage of transport per hour of RBE4 and hCMEC/D3 cells were measured 7 days after seeding

From: Similarities and differences in the localization, trafficking, and function of P-glycoprotein in MDR1-EGFP-transduced rat versus human brain capillary endothelial cell lines

Cell type TEER Mannitol permeability
(Ω cm2) (nm/s) (%/h)
RBE4-WT 15.55 ± 1.22 59.58 ± 4.07 4.20 ± 0.30
RBE4-MDR1 + dox 12.63 ± 0.54 60.69 ± 1.59 4.30 ± 0.22*
hCMEC/D3-WT 16.57 ± 1.06 53.76 ± 4.32 3.71 ± 0.13
hCMEC/D3-MDR1 + dox 15.79 ± 1.18 56.65 ± 2.52 3.81 ± 0.11
  1. Data are presented as the mean ± SEM of 3–8 replicates. Experiments were performed with ThinCert® cell culture inserts (12-well, 0.4 µm pore size, PET membranes). See “Materials and methods” and Additional file 1 for further details. Significant differences as calculated by one-way ANOVA (Bonferroni post hoc test) compared to hCMEC/D3-WT are marked by asterisk. All other values were not significantly different (P < 0.05)