Skip to main content

Table 1 TEER values and paracellular mannitol permeability (basolateral to apical) in nm/s and percentage of transport per hour of RBE4 and hCMEC/D3 cells were measured 7 days after seeding

From: Similarities and differences in the localization, trafficking, and function of P-glycoprotein in MDR1-EGFP-transduced rat versus human brain capillary endothelial cell lines

Cell type

TEER

Mannitol permeability

(Ω cm2)

(nm/s)

(%/h)

RBE4-WT

15.55 ± 1.22

59.58 ± 4.07

4.20 ± 0.30

RBE4-MDR1 + dox

12.63 ± 0.54

60.69 ± 1.59

4.30 ± 0.22*

hCMEC/D3-WT

16.57 ± 1.06

53.76 ± 4.32

3.71 ± 0.13

hCMEC/D3-MDR1 + dox

15.79 ± 1.18

56.65 ± 2.52

3.81 ± 0.11

  1. Data are presented as the mean ± SEM of 3–8 replicates. Experiments were performed with ThinCert® cell culture inserts (12-well, 0.4 µm pore size, PET membranes). See “Materials and methods” and Additional file 1 for further details. Significant differences as calculated by one-way ANOVA (Bonferroni post hoc test) compared to hCMEC/D3-WT are marked by asterisk. All other values were not significantly different (P < 0.05)
Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118