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Fig. 8 | Fluids and Barriers of the CNS

Fig. 8

From: Similarities and differences in the localization, trafficking, and function of P-glycoprotein in MDR1-EGFP-transduced rat versus human brain capillary endothelial cell lines

Fig. 8

Intracellular distribution of doxorubicin in RBE4 wildtype and -MDR1-EGFP cocultures. RBE4 wildtype and –MDR1-EGFP cocultures were stained for endolysosomal vesicles with LysoTracker (blue) and treated with the Pgp substrate doxorubicin (red) for 30 min, before live-cell imaging using a confocal laser scanning microscope. Insets show a magnification of endolysosomal localization of doxorubicin and Pgp (green). In RBE4 wildtype cells, doxorubicin binds to its nuclear target as visible by red staining (#1), while nuclei of Pgp-EGFP overexpressing cells lack a nuclear doxorubicin staining (#2). Similar experiments with similar outcomes were previously performed with cocultures of wildtype and MDR1-EGFP-transduced hCMEC/D3 cells [31], and therefore these experiments were not repeated and again illustrated here

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