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Fig. 7 | Fluids and Barriers of the CNS

Fig. 7

From: Similarities and differences in the localization, trafficking, and function of P-glycoprotein in MDR1-EGFP-transduced rat versus human brain capillary endothelial cell lines

Fig. 7

Directional P-glycoprotein transport assay in MDR1-EGFP expressing RBE4 and hCMEC/D3 cells. RBE4-MDR1-EGFP (A, B) and hCMEC/D3-MDR1-EGFP (C, D) were grown in the apical compartment of 12-well ThinCert® cell culture inserts, as well as MDR1-transfected LLC cells (E, F) for comparison. For measurement of directional Pgp-mediated transport by the concentration equilibrium transport assay (CETA), equal concentrations of the Pgp substrate [3H]dLop were added to the apical and basolateral compartment in the absence (A, C, E) or presence (B, D, F) of the Pgp inhibitor verapamil. Amounts of [3H]dLop in the apical and basolateral chamber were measured at various time points and presented as percent of initial drug concentration as mean ± SEM of 3 replicates. Data were analyzed by two-way (ANOVA) followed by Bonferroni post hoc tests. Asterisks show significant differences between the two chambers (P < 0.05)

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Fluids and Barriers of the CNS

ISSN: 2045-8118