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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Comparative assessment of in vitro BBB tight junction integrity following exposure to cigarette smoke and e-cigarette vapor: a quantitative evaluation of the protective effects of metformin using small-molecular-weight paracellular markers

Fig. 4

TSe and ECe impact of TJs expression and protective effect of metformin. The expression levels of the TJ proteins ZO-1(A) and occludin (B) were not significantly affected by exposure to TSe or ECe. However, claudin-5 expression was significantly downregulated by TSe and ECe (C). Note also that claudin-5 downregulation by TSe and ECe was abrogated by metformin pre-treatment. WB analyses report protein/β-actin ratios. **p < 0.01; ****p < 0.0001 vs. control. ANOVA; n = 3 biological replicates)

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Fluids and Barriers of the CNS

ISSN: 2045-8118