Fig. 2

Enhanced MMP activity after Lrp1 deletion increases permeability in cultured primary brain endothelial cells. A relative MMP activity, B transendothelial resistance and C ¹⁴C-inulin permeability of primary brain endothelial cells isolated from of 2-month-old Lrp1^lox/lox; Slco1c1-CreER^T2 mice and Lrp1^lox/lox littermate controls. Primary brain endothelial cells were cultivated on transwell inserts in the cellZcope device. Cells or supernatants were used for subsequent studies when confluent. TEER was 72.6 ± 2.53 for Lrp1^lox/lox and 56.69 ± 1.83 for Lrp1^lox/lox; Slco1c1-CreER^T2 endothelial cells. Mean ± SEM. B and C are biological replicates from 3 independent isolates. Significance was determined by Student’s t test