Fig. 1

Reduced levels of tight junction proteins and P-gp after Lrp1 CNS endothelial loss. Immunostaining for A LRP1, B claudin-5 and C ZO-1 in cultured endothelial cells of Lrp1^lox/lox; Slco1c1-CreER^T2 mice and Lrp1^lox/lox littermate controls. Scale bar: 20 µm. D Immunoblotting for occludin, and claudin 5. E cyclophillin A and F P-gp in isolated brain endothelial cells and their relative abundance compared with β-actin (loading control) of 2-month-old Lrp1^lox/lox; Slco1c1-CreER^T2 mice and Lrp1^lox/lox littermate controls. Mean ± SEM, n = 3 isolates/group. Significance was determined by Student’s t test