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Fig. 7 | Fluids and Barriers of the CNS

Fig. 7

From: Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size

Fig. 7

Representative images of NTE (black puncta) detecting i.v. injected a [125I]mAb3D6-scFv8D3 or b [125I]di-scFv3D6-8D3 and CD31-flourescent staining (red) in mouse brains. Black scale bar = 20 μm. c Quantification of NTE images for [125I]mAb3D6-scFv8D3 and [125I]di-scFv3D6-8D3-injected animals, the association of black puncta (antibody-derived signal) with parenchyma or capillaries in %area of black puncta. There was a significantly higher distribution to the capillaries of [125I]mAb3D6-scFv8D3 compared to [125I]di-scFv3D6-8D3 at all investigated time points, as determined by two way analysis of variance followed by Bonferroni’s post hoc test (****p < 0.0001). Values are reported as mean ± standard deviation, with 18–20 images for each time point and antibody. Brain pharmacokinetics corrected for parenchymal partitioning expressed as d %ID/g brain and e %ID/g brain corrected for body weight (bw) for [125I]mAb3D6-scFv8D3 and [125I]di-scFv3D6-8D3-injected animals. Values are reported as mean ± standard deviation

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