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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Microglia activated by microbial neuraminidase contributes to ependymal cell death

Fig. 3

Viability of isolated ependymal cells co-cultured with microglial cells. Ependymal cell cultures were co-cultivated with resting or stimulated (either with LPS or with NA) pure microglial cells. Viability of ependymocytes was assessed at different time points, up to 24 h, by trypan blue dye exclusion. Each point represents the percentage of living ependymocytes relative to the viability measured at the beginning of the experiment (100% viability at time 0). Data are the mean ± s.d. of n = 6 independent experiments, where ependymal and microglial cultures were obtained from different rats. Means of viability under different culture conditions were compared by two-way ANOVA, which showed significant differences between experimental conditions (P < 0.001) and between time points (P < 0.001). The Tukey test post hoc pointed out that, after 6 h of co-culture, the viability of ependymocytes co-cultured with microglia activated either with NA or with LPS was reduced (bottom bracket), compared to the viability measured in any of the other culture conditions used as controls (top bracket), which included ependymal cells alone, with LPS or with NA, or with not-activated microglia; this difference remained at 24 h. No differences in the viability of ependymocytes co-cultured with microglia were found when using LPS or NA to activate microglia (bottom bracket). Similarly, no differences were found between control groups (top bracket). Ep: ependymal cells, M: co-culture with microglial cells; (-): control medium; LPS: lipopolysaccharide added to medium; NA: neuraminidase added to medium. * = P < 0.001

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