Fig. 1
From: Microglia activated by microbial neuraminidase contributes to ependymal cell death
Purified microglia and ependymocyte cultures obtained for co-culture experiments. Pure microglial cultures visualized by bright field microscopy under control conditions (a) and after the addition of NA to the culture medium (d); NA induces a change in microglial morphology. Double immunofluorescence for IBA1 and the inflammatory cytokine IL-1β was performed on control (b, c) and NA-treated (e, f) microglia cultures. The identity and high purity (> 95%) of rat primary microglial cells was demonstrated by IBA1 fluorescence immunostaining (b, e). The activation of microglial cells after NA stimulation was evidenced by IL-1β labelling (c, f). Ependymal cells were likewise isolated from adult rats, and purified using an in vitro strategy. The purity (100% ependymal cells) [36] and identity of the isolated cells was evaluated by immunocytochemistry using the cilia specific marker βIV-tubulin (g, h). The bunch of cilia in ependymal cells is noticeable (h). Scale bars in a–h: 10 μm