Fig. 5

AC HIF-1 KD does not alter EC migration. a Representative images of scratched confluent EC monolayers at 0 h and after 6 h exposure. Scale bar = 100 μm. EC were scratched and subsequently treated with RBE4 media or normoxic and hypoxic AC-CM, then exposed to normoxia or hypoxia for 6 h or 24 h. After quantification mean migration is graphed as µm/h for b 6 h and c 24 h. 2way ANOVA, mean ± SD, n = 3, *p < 0.05, **p < 0.01 ***p < 0.001, ****p < 0.0001 compared to RBE4 media Nx, ^ϕp < 0.05, ^ϕϕp < 0.01, ^ϕϕϕp < 0.001 to RBE4 media Hx