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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Astrocyte-specific hypoxia-inducible factor 1 (HIF-1) does not disrupt the endothelial barrier during hypoxia in vitro

Fig. 1

Schematic of AC-CM experiments. Graphical overview of experimental setup using primary AC (dark green) and the RBE4 EC cell line (red). Media was collected from normoxic (white) or hypoxic untreated (UNT, teal), scrambled transfected (scram, grey) or siHIF-1α transfected AC (si-HIF1α, teal-dashed) and snap frozen before use. Thawed AC-CM was transferred to confluent RBE4 immediately before exposure. All experiments were compared to RBE4 grown in their own culture media (RBE4 media, red)

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