Fig. 5

CLDN5 levels in CSF correlates with brain-injury severity 24 h after HI. The grey matter tissue loss, defined as the average percentage of negative MAP2 immunoreactivity calculated from six levels throughout the whole brain, correlates with the levels of CLDN5 in the CSF of the same animal 24 h post-HI (a) with a Pearson correlation coefficient (r) of 0.702 (p = 0.016). No correlation was observed between the loss of grey matter and levels of CLDN5 in plasma (c) nor OCLN in CSF (b) or plasma (d). The tissue loss varied between 24.7 to 59.9%, three representative images of injured brain hemispheres are shown in (e), n = 12 (5 m, 7f). Scale-bars are two mm in all images. m  male, f  female