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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Circulating tight-junction proteins are potential biomarkers for blood–brain barrier function in a model of neonatal hypoxic/ischemic brain injury

Fig. 4

CLDN5 immunoreactivity is not altered in the cerebral blood vessels of neonatal rats after HI. Representative double-immunoreactivity for CD31 (red, blood vessel marker) and tight-junctional proteins CLDN5 (green) in cortical brain sections collected 6 h after hypoxia/ischemia (a). Scale-bars are 100 μm in all images. No difference in CLDN5-coverage were seen (b) between controls or either hemisphere in HI-animals at 6 h (2 m, 1f), 24 h (2 m, 1f), 5d (2 m, 1f) post-HI (left in b; p > 0.5) nor between the injured and uninjured hemisphere in HI animals (right in b; p > 0.05). HI, n = 3 per time point, controls (n = 2 per time-point). One-way ANOVA with Dunnett’s multiple comparison test and paired t-tests. m  male, f female. Bar graphs represent mean values

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