Fig. 2

Principal component analysis discriminate between HI and control animals. Discriminate analysis (principal component analysis-plots) using CLDN5- and OCLN-measurements in plasma and CSF at different times after HI. Plots include 6 h (n = 7) and 24 h HI (n = 7) as well as 6 and 24 h controls (n = 6) which groups in three distinct groups in the analysis (a), this grouping in lost when analysing 5 days after HI (n = 7) and 5 days controls (n = 10) (b). Pooling all samples and adjusted for time as a factor (c) male rats (n = 10) were shown to have significantly higher levels of OCLN in plasma (p = 0.035) and CLDN5 in CSF (p = 0.036) than female rats (n = 11). No sex-difference were seen in plasma OCLN (p = 0.91) or CSF CLDN5 (p = 0.19) in control animals (males n = 7, females n = 9). Unpaired t-test, box-plots showing median, quartiles, and range (whiskers)