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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

Fig. 6

Transwell permeability assay. a Apparent permeability coefficient (P_app) of HBMECs cultured for 2 days with 0d or 1d cAMP treatment. N = 6, where N is the number of inserts measured over 3 trials. Inserts were analyzed using the JAnaP for ZO-1 (b) and VE-cadherin (c), and the correlation between junction coverage and P_app were evaluated (d, e). A linear regression rendered the slope of all relationships non-significantly non-zero. For (b, c), 232 ≤ N ≤ 244, where N is the number of cells, and for (d, e), N = 12, where N is the number of inserts pooled between the 0d and 1d cAMP conditions. The Mann–Whitney test was used to calculate significant differences for each parameter, where ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001

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Fluids and Barriers of the CNS

ISSN: 2045-8118