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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

Fig. 5

Immunofluorescence images and junction phenotype analysis for claudin-5. HBMECs on Fbn, cultured for a 4-days and b 7-days, with 0, 1, 3, or 6 days of cAMP treatment, stained for claudin-5 (green), VE-cadherin (red), and DNA (blue) (scale bar = 20 μm). Edge presentation of continuous, punctate, and perpendicular junctions for claudin-5 for c 4-day (19 ≤ N ≤ 47) and d 7-day culture (21 ≤ N ≤ 52), where N is the number of cells. The Kruskal–Wallis test with a Dunn’s multiple comparison test was used to calculate significant differences, where ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001

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