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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

Fig. 4

Immunofluorescence images and junction phenotype analysis for 7-day culture. a Schematic representing treatment schedule for 7-day experiment. b HBMECs on Fbn, cultured for 7 days 0, 1, 3, or 6 days of cAMP treatment, stained for ZO-1 (green), VE-cadherin (red), and DNA (blue). (scale bar = 20 μm) Edge presentation of continuous, punctate, and perpendicular junctions for c ZO-1 and d VE-cadherin. 74 ≤ N ≤ 115, where N is the number of cells. The Kruskal–Wallis test with a Dunn’s multiple comparison test was used to calculate significant differences, where ns = p > 0.05, *p < 0.05, **p < 0.01, and ****p < 0.0001

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Fluids and Barriers of the CNS

ISSN: 2045-8118