Skip to main content
Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

Fig. 3

Immunofluorescence images and junction phenotype analysis for 4-day culture. a Schematic representing treatment schedule for 4-day experiment. b HBMECs on Fbn, cultured for 4 days with 0, 1, or 3 days of cAMP treatment, stained for ZO-1 (green), VE-cadherin (red), and DNA (blue). (scale bar = 20 μm) Edge presentation of continuous, punctate, and perpendicular junctions for (c) ZO-1 and (d) VE-cadherin. 87 ≤ N ≤ 145, where N is the number of cells. The Kruskal–Wallis test with a Dunn’s multiple comparison test was used to calculate significant differences, where ns = p > 0.05, ***p < 0.001, and ****p < 0.0001

Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118