Skip to main content
Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

Fig. 2

Immunofluorescence images and junction phenotype analysis for 2-day culture. a Schematic representing treatment schedule for 2-day experiment. b HBMECs on 6 substrate coatings, cultured for 2 days with and without cAMP treatment, stained for ZO-1 (green), VE-cadherin (red), and DNA (blue). (scale bar = 20 μm) Edge presentation of continuous (c, f), punctate (d, g), and perpendicular (e, h) junctions for ZO-1 and VE-cadherin, respectively. 72 ≤ N ≤ 125, where N is the number of cells. The Kruskal–Wallis test with a Dunn’s multiple comparison test was used to calculate significant differences, where *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. See Additional file 1: Table S2 for statistical comparison between each protein coating

Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118