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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

Fig. 1

JAnaP workflow. a Example cell of interest within a monolayer immunostained for ZO-1. b Isolated cell junctions after the filtering and threshold value has been applied. c Example cells depicting solidity (orange) and circularity (gray) calculations, where A and P are the cell area and perimeter (white outline), and Aconvex is the convex area (yellow outline). d Criteria for junction categorization to differentiate between continuous (cont., blue), punctate (punct., magenta), and perpendicular (perp., light blue) junctions. ef Categorized junctions for the cell of interest, where (e) also depicts the perimeter of the cell (thin white line) and a cropped image of the dotted white region to improve visibility of the junctions (scale bar = 20 μm)

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