Table 1 List of primary antibodies
Antigen | Immunogen | Manufacturer (+CAT#) | Host and isotype | Dilution | Reference RRID# |
|---|---|---|---|---|---|
AQP1 | (C)KVWTSGQVEEYDLDADDIN Target epitope in mouse AQP1 are amino acids 241 to 261 intracellular | Alomone Labs Cat# AQP-001 | Rabbit IgG | 1:400 | AB_2039726 |
AQP1 | 19-aa peptide of Rat AQP1 Target epitope is in C-terminus, intracellular | Alpha Diagnostic Cat# AQP11-A | Rabbit IgG | 1:800 | AB_1609286 |
AQP1 | (C)SAVLTRNFSN Target epitope in mouse AQP1 are amino acids 199 to 208 extracellular | Abcam Cat# ab11025 | Mouse IgG2b | 1:500 | AB_2289651 |
NKCC1 | Synthetic peptide derived from the N terminal region of human NKCC1 target epitope located intracellularly | Abcam Cat# ab59791 | Rabbit IgG | 1:500 | AB_944433 |
NKCC1 | (C)RRQAMKEMSIDQAK Target epitope in mouse NKCC1 are amino acids 821 to 834 extracellular | Alomone Labs Cat# ANT-071 | Rabbit IgG | 1:100 | AB_2341018 |
α-SMA | Synthetic peptide corresponding to human alpha smooth muscle Actin (N terminal) | Abcam Cat# ab7817 | Mouse IgG2a | 1:500 | AB_262054 |
CD31 | Tissue, cells or virus corresponding to human CD31 | Abcam Cat# ab24590 | Mouse IgG | 1:500 | AB_448167 |
GAPDH | Source: rabbit muscle (commercially sensitive) | Sigma Aldrich Cat# G8795 | Mouse IgM | 1:5000 | AB_1078991 |