Fig. 4

Resistance to CSF drainage values in female rats fed a C or HF diet with cytokine treatment. Each VRI experiment was carried out by perfusing aCSF with each treatment through one lateral ventricle of the rat brain. Values were calculated as a percentage change from each respective control from female C (n = 3) and female HF (n = 3) rats for each respective cytokine treatment (n = 3). The graph shows the averaged resistance to CSF drainage readings (mmH₂O min/μl) as a percentage change from control. A two-way ANOVA was used to analyse the statistical significance. The significant results are shown following Sidak’s multiple comparison test and was performed for each diet and treatment variable. ***P = ≤ 0.001, ****P = ≤ 0.0001. aCSF artificial cerebrospinal fluid, C control diet, HF high-fat diet, VRI variable rate infusion, HC hydrocortisone, TNF-α tumour necrosis factor-α, IL-6 interleukin-6, IL-17 interleukin-17, CCL2 C–C motif chemokine ligand-2