Fig. 1
From: Disparate volumetric fluid shifts across cerebral tissue compartments with two different anesthetics
Experimental design of the study and overview of computational analysis. a The experiment was designed as cross-over repeated measures study. Twelve rats underwent four MRI scan sessions under two different anesthetic regimens at least 7 days apart to ensure adequate wash-out effects of each anesthetic. b Each B1 corrected proton-density weighted (PDW) image was spatially registered and segmented into grey matter (GM), white matter (WM), and CSF using custom made tissue probability maps. Subsequently the images were spatially normalized using deformation fields and processed with voxel-wise analyses. Similarly, each T2* image was spatially normalized using deformation fields derived from PDW images and evaluated by voxel-wise analyses. c Apparent diffusion coefficient (ADC) maps were calculated at low-, mid-, and high-evel b-factors in addition to kurtosis maps; and analyzed by both voxel-wise and regions of interest analyses