Fig. 5

PSEN mutants display an increased Aβ1–40 secretion and intracellular concentrations. a Aβ1–40 secretion levels in the cell conditioned medium. Cells were allowed to condition in such medium for 24 h before harvesting. Note the extremely high levels in both PSEN1-BMECs and PSEN2-BMECs compared to control. Secreted Aβ1–42 was below detection limits for all three cell lines (data not shown). b Aβ1–40 and Aβ1–42 in cell homogenates. Note the presence of both Aβ1–40 and 1–42 in PSEN1-BMECs, at an amount significantly higher than control. N = 3/group, * and ** denote P < 0.05 and P < 0.01 versus the control group. # and ## denote P < 0.05 and P < 0.01 between PSEN1 and PSEN2 groups