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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Presence of a mutation in PSEN1 or PSEN2 gene is associated with an impaired brain endothelial cell phenotype in vitro

Fig. 1

PSEN1-BMECs show impaired tight junctions and barrier function. a Representative micrograph picture of claudin-5, occludin, and ZO-1 immunofluorescence in iPSC-derived BMECs differentiated from control, PSEN1, and PSEN2 patients. Scale bar = 50 µm. b Semi-quantitative analysis of claudin-5 and occludin protein expression. Protein expression was quantified using fluorescence intensity obtained in random fields. Note the relative lower expression of claudin-5 and occludin in the PSEN1 group compared to PSEN2 group. c TEER and d fluorescein permeability values in iPSC-derived BMECs at day 10 of differentiation. Note the poor TEER value and higher permeability in the PSEN1 group compared to other groups. N = 3/group, ** Denotes P < 0.01 compared to controls

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Fluids and Barriers of the CNS

ISSN: 2045-8118