Fig. 2
From: Cerebrospinal fluid drainage kinetics across the cribriform plate are reduced with aging
Ex vivo images of CSF outflow regions show mainly nasal and spinal routes. Representative images of the dorsal a and ventral b brain surfaces revealed retention of tracer along blood vessels, fissures and brain surfaces after 150 min. c Quantification of intensities on the dorsal and ventral surface using the same imaging and analysis parameters as in the in vivo studies. Representative images of the dorsal d and ventral e skull cap surfaces with intact dura, spinal cord (f; C (cervical) and S (sacral)), and superficial (sLN; g) and deep (dLN; h) cervical lymph nodes (white arrow heads) and afferent and efferent lymphatic vessels (red arrow heads) in the exposed neck region. i Quantification of NIRF intensities in the sLN and dLN. NIRF images were enhanced equally to show all possible locations of NIRF-Albumin after 150 min. CF (circular fissure), LF (longitudinal fissure), TF (transverse fissure), ACA (anterior cerebral artery), MCA (middle cerebral artery), PCA (posterior cerebral artery), BA (basilar artery), FS (frontonasal suture), SS (sagittal sinus), TS (transverse). j Quantification of intensity for the dorsal and ventral brain surfaces 30 min post-injection. k Comparison of dorsal to ventral intensity ratios at 30 and 150 min. Representative Albumin-Evans blue (EB) bright field images after 30 min for the ventral brain surface with intact skull cap (l) and the cut surface across around the cribriform plate region (m) at the white line in panel L. OPC (optic nerve chiasm), Trig N (trigeminal nerve) and Crib P (cribriform plate area). Residual pigment from the eyes is shown at the top and residual EB at the bottom. Values are mean ± SD, N = 5 young male mice. Scale bar: 5 mm