Fig. 4
From: Identification and in vivo characterization of a brain-penetrating nanobody
Brain-penetrating efficiency of Nb62 mutants. a Binding curves of anti-mTfR nanobodies to immobilized mTfR by ELISA (n = 4). Data were fitted by nonlinear regression using the GraphPad Prism 8 software (San Diego, CA, USA). b TLR4−/− mice body temperature measurements after IV injections of Nb62 and mutants fused to NT (8–13) at a dose of 250 nmol/kg (****p < 0.0001). Bar graphs represent mean ± SEM (n = 3 per group). Statistical test: two-way ANOVA with Dunnett multiple comparisons test compared to negative control nanobody