Fig. 2
From: Identification and in vivo characterization of a brain-penetrating nanobody
Evaluation of anti-mTfR nanobodies for receptor-mediated transcytosis. a Theoretical pIs of the anti-mTfR nanobodies fused to NT, with or without an incorporated acidic peptide, calculated based on their primary sequence by CLC Main Workbench 8.1. A pI corresponding to no net charge under physiological conditions is indicated by the red dotted line. b TLR4−/− mice body temperature measurements after 250 nmol/kg intravenous injections of the indicated nanobody fused to NT. c TLR4−/− mice body temperature measurements after 5 pmol ICV injection of the negative control nanobody fused to NT. d TLR4−/− mice body temperature measurements after 250 nmol/kg IV injections of Nb62 or negative control (**p = 0.004). Bar graphs represent mean ± SEM (n = 3 per group, p = 0.004). Statistical test: two-way ANOVA with Dunnett multiple comparisons test compared to negative control nanobody