Fig. 6

iBMECs are responsive to shear stress on LN 511-E8. a–c Phase contrast images of iBMECs before the start of flow (a) or under static (b) and dynamic (c) conditions for 24 h. White arrow indicates the flow direction. Scale bars indicate 200 μm. d–f Quantification of cellular shape factors including circularity (c), inverse of aspect ratio (d), average cell surface area (e) for iBMECs cultured under static or dynamic conditions for 24 h. Median with interquartile range are shown, and * indicates P < 0.0001 compared to static conditions. Data are representative of two biological replicates, each with 6 images analyzed per condition from three different devices. g Immunocytochemical analysis of iBMECs cultured for 24 h under static conditions followed by 24 h of static (top row) or dynamic (bottom row) conditions shows improvement in junctional expression of ZO-1 and VE-cadherin and the formation of stress fibers under dynamic conditions. Scale bars indicate 200 μm