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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Chemically defined human vascular laminins for biologically relevant culture of hiPSC-derived brain microvascular endothelial cells

Fig. 4

Differences in endothelial cell morphology on CN IV-FN and LN511-E8. a Schematic representation of iBMEC morphology on CN IV-FN (left) and LN 511-E8 (right). b, c Phase contrast images of iBMECs cultured on CN IV-FN (b) or LN511-E8 (c) 4 days after subculture. Scale bars indicate 400 μm. d, e Maximum intensity projections of confocal z-stack images of claudin-5 staining in cell culture inserts 4 days after subculture on CN IV-FN (d) or LN 511-E8 (e). f, i Three-dimensional representations of the z-stack images showing the difference in junction height on CN IV-FN (f, h) compared to LN 511-E8 (g, i)

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Fluids and Barriers of the CNS

ISSN: 2045-8118