Fig. 1

TEER measurement results for iBMECs differentiated from IMR-90-4 hiPSCs after subculture on different ECM proteins. a Schematic of iBMEC purification and cell seeding on different protein-coated surfaces. b TEER results for the purified iBMECs subcultured on different ECM protein-coated inserts for 5 days. **indicates P < 0.001 compared to CN IV-FN group. Differences between other groups are not statistically significant. c TEER measurement to evaluate long-term stability of the barrier after subculture onto LN 511-E8, LN 411-E8, or CN IV-FN. **indicates P < 0.001 compared to CN IV-FN group. Differences between LN 411-E8 and CN IV-FN samples are not statistically significant. A two-factor repeated measures ANOVA was used to analyze TEER data in (b) and (c). d Percent reduction in TEER compared to the peak TEER (TEER value on day 2) for the results shown in (c), indicating formation of a more stable barrier for iBMECs cultured on LN 511-E8. * indicates P < 0.05 and ** indicates P < 0.001 compared to CN IV-FN samples on the same day