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Table 2 Comparison of transendothelial electrical resistance (TEER) and paracellular mannitol permeability in hCMEC/D3-WT, hCMEC/D3-Cldn5-YFP and pBCECs

From: A face-to-face comparison of claudin-5 transduced human brain endothelial (hCMEC/D3) cells with porcine brain endothelial cells as blood–brain barrier models for drug transport studies

  TEER (Ω cm2) Mannitol permeability
Chopstick EndOhm-6 chamber nm/s %/h
hCMEC/D3-WT 117 ± 9 10.9 ± 1.6 111 ± 5 14.2 ± 0.4
hCMEC/D3-Cldn5-YFP 211 ± 8* 29.6 ± 1.6* 80.5 ± 0.7* 8.06 ± 0.1*
pBCEC 1650 ± 46# 1141 ± 86# 23.3 ± 5# 3.95 ± 0.6#
  1. Data are presented as the mean ± SEM of 3 replicates. For TEER, which was measured by either chopstick electrodes or an EndOhm-6 tissue resistance measurement chamber, significant difference between hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by asterisk (P < 0.01), whereas significant difference between pBCECs versus hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by the hash sign (P < 0.001). For mannitol values, significant difference between hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by asterisk (P = 0.0040), whereas significant difference between pBCECs versus hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by the hash sign (P < 0.001)