| |
TEER (Ω cm2)
|
Mannitol permeability
|
|---|
|
Chopstick
|
EndOhm-6 chamber
|
nm/s
|
%/h
|
|---|
|
hCMEC/D3-WT
|
117 ± 9
|
10.9 ± 1.6
|
111 ± 5
|
14.2 ± 0.4
|
|
hCMEC/D3-Cldn5-YFP
|
211 ± 8*
|
29.6 ± 1.6*
|
80.5 ± 0.7*
|
8.06 ± 0.1*
|
|
pBCEC
|
1650 ± 46#
|
1141 ± 86#
|
23.3 ± 5#
|
3.95 ± 0.6#
|
- Data are presented as the mean ± SEM of 3 replicates. For TEER, which was measured by either chopstick electrodes or an EndOhm-6 tissue resistance measurement chamber, significant difference between hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by asterisk (P < 0.01), whereas significant difference between pBCECs versus hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by the hash sign (P < 0.001). For mannitol values, significant difference between hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by asterisk (P = 0.0040), whereas significant difference between pBCECs versus hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by the hash sign (P < 0.001)
