Day after seeding
|
TEER (Ω cm2)
|
---|
hCMEC/D3-WT
|
hCMEC/D3-Cldn5-YFP
|
---|
1
|
10.1 ± 0.8
|
15.2 ± 1.2*
|
2
|
14.8 ± 0.8
|
22.6 ± 0.8*
|
3
|
18.7 ± 0
|
26.5 ± 1.5*
|
4
|
19.5 ± 0.8
|
26.5 ± 1.5*
|
5
|
18.7 ± 0
|
26.5 ± 1.5*
|
6
|
16.3 ± 2.3
|
24.9 ± 1.5*
|
7
|
15.5 ± 1.5
|
26.5 ± 1.5*
|
8
|
16.3 ± 2.3
|
28.0 ± 0*
|
9
|
17.9 ± 2.1
|
26.5 ± 1.5*
|
- Data are presented as the mean ± SEM of 3 replicates. TEER was measured utilizing an EndOhm-6 tissue resistance measurement chamber. Significant difference between hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by asterisk (P < 0.05). This experiment is a repeat of the experiment shown in Fig. 4b, but TEER measurements were performed up to 9 days after seeding