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Table 1 Comparison of transendothelial electrical resistance (TEER) in hCMEC/D3-WT vs. hCMEC/D3-Cldn5-YFP cells

From: A face-to-face comparison of claudin-5 transduced human brain endothelial (hCMEC/D3) cells with porcine brain endothelial cells as blood–brain barrier models for drug transport studies

Day after seeding

TEER (Ω cm2)

hCMEC/D3-WT

hCMEC/D3-Cldn5-YFP

1

10.1 ± 0.8

15.2 ± 1.2*

2

14.8 ± 0.8

22.6 ± 0.8*

3

18.7 ± 0

26.5 ± 1.5*

4

19.5 ± 0.8

26.5 ± 1.5*

5

18.7 ± 0

26.5 ± 1.5*

6

16.3 ± 2.3

24.9 ± 1.5*

7

15.5 ± 1.5

26.5 ± 1.5*

8

16.3 ± 2.3

28.0 ± 0*

9

17.9 ± 2.1

26.5 ± 1.5*

  1. Data are presented as the mean ± SEM of 3 replicates. TEER was measured utilizing an EndOhm-6 tissue resistance measurement chamber. Significant difference between hCMEC/D3-WT and hCMEC/D3-Cldn5-YFP cells is indicated by asterisk (P < 0.05). This experiment is a repeat of the experiment shown in Fig. 4b, but TEER measurements were performed up to 9 days after seeding
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Fluids and Barriers of the CNS

ISSN: 2045-8118