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Table 1 Immunohistochemical markers used to investigate ventricular zone and stem cells biology

From: Preterm intraventricular hemorrhage in vitro: modeling the cytopathology of the ventricular zone

Antibody

Host species

Dilution IHC

Dilution WB

Source

GFAP

Rabbit

1:300

1:1000

#7260, Abcam

βIV tubulin

Mouse

1:100

–

#11315, Abcam

AQP4

Rabbit

1:1000

–

#PAB 20767 Abnov

β-catenin

Mouse

1:100

–

#Ab16051 Abcam

S100β

Rabbit

1:100

–

#Ab52642, Abcam

N-cadherin

Mouse

1:25

1:50

#180224, Thermo Fisher Scientific

EMA

Mouse

1:100

–

#790-4463, Roche

GAPDH

Rabbit

–

1:500

#2118S, Cell Signaling Technology

Î’-actin

Mouse

–

1:500

#A1978 Sigma

Antimouse Alexa fluor 555

Goat

1:300

–

#AQ21422, Thermo Fisher Scientific

Antirabbit Alexa Fluor 488

Goat

1:300

–

#A11034, Thermo Fisher Scientific

Peroxidase-conjugated antirabbit

–

–

1:10,000

#70,742, Cell Signaling Technology

Peroxidase-conjugated antimouse

–

–

1:5000

#SC-2005, Santa Cruz Biotechnology

  1. Abcam, Cambridge, UK
  2. Abnova, Taipei, Taiwan
  3. Thermo Fisher Scientific, Waltham, MA
  4. Cell Signaling Technology, Danvers, MA
  5. Santa Cruz Biotechnology, Dallas, TX