From: Preterm intraventricular hemorrhage in vitro: modeling the cytopathology of the ventricular zone
Antibody | Host species | Dilution IHC | Dilution WB | Source |
---|---|---|---|---|
GFAP | Rabbit | 1:300 | 1:1000 | #7260, Abcam |
βIV tubulin | Mouse | 1:100 | – | #11315, Abcam |
AQP4 | Rabbit | 1:1000 | – | #PAB 20767 Abnov |
β-catenin | Mouse | 1:100 | – | #Ab16051 Abcam |
S100β | Rabbit | 1:100 | – | #Ab52642, Abcam |
N-cadherin | Mouse | 1:25 | 1:50 | #180224, Thermo Fisher Scientific |
EMA | Mouse | 1:100 | – | #790-4463, Roche |
GAPDH | Rabbit | – | 1:500 | #2118S, Cell Signaling Technology |
Β-actin | Mouse | – | 1:500 | #A1978 Sigma |
Antimouse Alexa fluor 555 | Goat | 1:300 | – | #AQ21422, Thermo Fisher Scientific |
Antirabbit Alexa Fluor 488 | Goat | 1:300 | – | #A11034, Thermo Fisher Scientific |
Peroxidase-conjugated antirabbit | – | – | 1:10,000 | #70,742, Cell Signaling Technology |
Peroxidase-conjugated antimouse | – | – | 1:5000 | #SC-2005, Santa Cruz Biotechnology |