Fig. 7

IGF1R protein expression in brain microvessels (BMVs). a Wes™ band density of IGF1R expression in human (h) and mouse (m) BMVs. b Quantification of IGF1R expression by Wes™. IGF1R in each lane is normalized to β-actin in the same lane; the fold-change of human IGF1R is relative to mouse IGF1R (set as one). Bars are mean ± SD of three biological replicates. The level of mIGF1R is significantly than hIGF1R (p < 0.0001; unpaired two-tailed t-test). c–e IGF1R (red) immunofluorescence in mouse BMV. f–g IGF1R immunofluorescence in human BMVs. Nuclei were stained with Hoechst (blue). The scale bar is 20 µm (unless indicated differently in the micrographs). h IGF1R expression (brown) in human BMVs detected by immunohistochemistry. i Negative control (no primary antibody) for Panel H. Scale bar for H and I is 10 µm