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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Differential expression of receptors mediating receptor-mediated transcytosis (RMT) in brain microvessels, brain parenchyma and peripheral tissues of the mouse and the human

Fig. 3

The enrichment of transcript for specific cell types in isolated mouse and human brain microvessels (BMV) compared to whole brain. BMVs and whole brain extracts were isolated as described in Materials and Methods and subjected to RNASeq analyses. aEndothelial cell/BBB markers: tight-junction protein 1 (TJP1)/ZO1, Occludin (OCLN), VE-cadherin, transporters (GLUT1, ABCG2), enzymes (ALP, γ-GTPase), and endothelial cell markers (E-selectin, CD31). bAstrocyte-specific markers: GLAST1 and SLC1A2; Pericyte–specific markers: PDGFRB, DES; ACTA2, ANPEP in human and mouse BMVs and total brain tissue. Each bar represents three biological replicates (Mean ± SEM). The enrichment of a gene transcript in mouse BMV vs. whole brain and in human BMV vs. whole brain was analyzed by two-tailed student t-test. Data are shown as Log2 ratios of various genes between levels in vessels and total brain

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Fluids and Barriers of the CNS

ISSN: 2045-8118