Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells

Table 8 Top differentially expressed pathways for the comparison of soloculture vs. coculture with bovine pericytes (CBP) at 24 h

Term	Database	Corrected p-value
Osteoclast differentiation	KEGG	8.46E−04
Regulation of insulin-like growth factor (IGF) transport and uptake by insulin-like growth factor binding proteins (IGFBPs)	Reactome	0.001
Cytokine Signaling In Immune System	Reactome	0.002
Syndecan interactions	Reactome	0.003
Extracellular matrix organization	Reactome	0.004
TNF signaling pathway	KEGG	0.004
Notch signaling pathway	PANTHER	0.006
Immune system	Reactome	0.006
Cell surface interactions at the vascular wall	Reactome	0.012
Non-integrin membrane-ECM interactions	Reactome	0.014
Interferon alpha/beta signaling	Reactome	0.017
Interferon signaling	Reactome	0.019
Hemostasis	Reactome	0.020
ECM proteoglycans	Reactome	0.022
Platelet degranulation	Reactome	0.022
Response to elevated platelet cytosolic Ca2+	Reactome	0.024
Nicotine degradation	PANTHER	0.028
Molecules associated with elastic fibers	Reactome	0.029
Integrin cell surface interactions	Reactome	0.030
Tryptophan metabolism	KEGG	0.031
Hepatitis B	KEGG	0.032
PECAM1 interactions	Reactome	0.040
Elastic fiber formation	Reactome	0.041
AGE-RAGE signaling pathway in diabetic complications	KEGG	0.046
GRB2/SOS provides linkage to MAPK signaling for Integrins	Reactome	0.047

Pathway analysis was conducted using different databases i.e. KEGG pathway, Reactome and Panther [37]. Statistical analysis was performed using the Fisher’s exact test and false discovery rate correction test was performed by the Benjamini and Hochberg method [3]

ISSN: 2045-8118