Fig. 5
From: Altered brain fluid management in a rat model of arterial hypertension
Visualisation and quantification of AQP4 expression in the cortex. a Representative images of AQP4 (green) and myosin (red) immunostaining in the cortex of normotensive (WKY) and spontaneously hypertensive (SHR) rats. Cell nuclei are visualized using DAPI staining (blue). b AQP4 fluorescence intensity profile as function of the distance from the luminal side of the vessel wall. Shown is the mean intensity profile with SEM bands for arteriole, capillary, and venule for both strains. c The area under the curve (AUC) of the AQP4 intensity profile of arterioles was significantly higher compared to capillaries and venules. In addition, venules showed significantly higher AUC values when compared to capillaries. AUC values of either arterioles, capillaries or venules did not differ between the two groups. d Total AQP4 expression in the cerebral cortex was not different between WKY (n = 11) and SHR (n = 10). The boxplots indicate the median and values of the 25th and 75th percentile of the data. ***p ≤ 0.001 (two-way ANOVA with Bonferroni’s post hoc tests for the AUC and Mann–Whitney U test for the total AQP4 expression analysis). Scale bar represents 100 µm