Fig. 4

P-glycoprotein efflux activity in NVUs. Data in a were acquired with primary human BMECs cultured for 8 days prior to measurements. Data in b were acquired with iPSC-derived BMECs cultured for 2 days prior to measurements. For all experiments, astrocyte culture was initiated on day 5 for primary BMEC and day 1 for iPSC-derived BMECs. a Inhibition of P-glycoprotein in primary human BMECs via LSN335984 increases loperamide permeability regardless of culture format. Data represent mean ± S.E.M. from N = 5 biological replicates. Statistical significance was calculated using the student’s unpaired t-test (*p < 0.05; **p < 0.005). b Inhibition of P-glycoprotein in iPSC-derived BMECs via cyclosporin A (CsA) increases rhodamine permeability in NVUs. Data represent mean ± S.E.M. from N = 5 biological replicates. Statistical significance was calculated using the student’s unpaired t-test (*p < 0.05)