Fig. 3

Permeability comparisons in primary human BMECs seeded in NVUs or Transwell setups. a Explicit permeability calculations for 3 kDa dextran extravasation through primary human BMECs in NVUs. The top panel contains the full dataset, while the bottom panel is the magnified inset to highlight the permeability values on the y-axis at later time points. Data represent mean ± S.E.M. from N = 10 biological replicates. b 3 kDa dextran permeability comparison between primary human BMECs seeded in NVUs versus Transwell filters. Measurements were acquired on day 8 after BMECs were seeded in each platform. Data represent mean ± S.E.M. from N = 10 biological replicates. Statistical significance was calculated using the student’s unpaired t-test (**, p < 0.01). c Comparison of size-dependent dextran permeability between primary human BMECs seeded in NVUs versus Transwell filters. Measurements were acquired on day 8 after BMECs were seeded in each platform. The corresponding table notes the size of each dextran and its corresponding fluorophore. The table also notes the standard error from each experimental system. Data in the bar chart represent mean ± S.E.M. from N = 10 biological replicates. Statistical significance was calculated using the student’s unpaired t-test (*p < 0.05; **p < 0.01). For all experiments, astrocyte co-culture was initiated on day 1