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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Advances in blood–brain barrier modeling in microphysiological systems highlight critical differences in opioid transport due to cortisol exposure

Fig. 2

Timeline for barrier formation in NVUs seeded with primary human BMECs. a Time course for barrier formation in the new NVU design as measured by 3 kDa dextran extravasation. Each curve represents a single NVU device (N = 8 biological replicates). The top panel contains the full dataset, while the bottom panel is the magnified inset to highlight the permeability values on the y-axis at later time points. b Explicit comparison of barrier formation between the original and new NVUs. The first red circle indicates when primary human astrocytes were added to the brain chamber. The second red circle indicates when iPSC-derived cortical neurons were added to the brain chamber. Because neurons did not improve barrier properties, they were not utilized in further experiments. Data represent mean ± S.E.M. from N = 10 biological replicates

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Fluids and Barriers of the CNS

ISSN: 2045-8118