Fig. 6

Light and dark variation in ICP: ICP values from continuously measured rats during the recovery period (RP) and physiological period (PP). a, b Difference in ICP in dark phase compared to light (0.0) in RP, showing the mean ICP for the whole period. c Trace detailing light and dark ICP measurements in the PP. In a and c, circular points represent dark phase values and square points represent light phase values. d The difference in ICP during dark phase (active phase) (combined (n = 10), single-housed group (n = 4) and co-housed group (n = 6)) relative to the ICP in the light phase (inactive phase). The dotted line represents zero change. E) Mean ICP in single-housed versus co-housed animals. Paired t-test for B, ANOVA followed by post hoc Dunn’s test for D and unpaired t-test for E. n = 10 and data presented as mean ± SEM