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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells

Fig. 4

The effect of TGF-β inhibitor on freezing–thawing of BMECs. a Freezing–thawing was performed at day 8 and the TEER values were measured at day 10. Statistical significance was calculated using the unpaired Student’s t-test (**p < 0.01). Data are represented as the mean ± SD (n = 3). b Temporal changes in TEER values were observed in the BMECs with TGF-β inhibitor. Statistical significance was calculated using two-way repeated measures ANOVA. Data are represented as the mean ± SD (n = 3). c Immunofluorescence for the tight junction markers (ZO-1 and occludin). Blue: DAPI. White arrows: discontinuous tight junctions. Scale bar, 50 μm. d Mean fluorescence intensities of P-gp and BCRP staining were calculated. Statistical significance was calculated using the unpaired Student’s t-test, non-frozen of control = 1. Data are represented as the mean ± SD (n = 3). e Relative gene expression levels of MDR1, BCRP, GLUT1, VE-cadherin, occludin, and ZO-1. Statistical significance was calculated using the unpaired Student’s t-test (*p < 0.05), non-frozen = 1. Data are represented as the mean ± SD (n = 3)

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Fluids and Barriers of the CNS

ISSN: 2045-8118