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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells

Fig. 3

Protein expression and activities of efflux transporters in iBMELCs. a Immunofluorescence and mean fluorescence intensity of transporters (GLUT1, BCRP, and P-gp). Blue: DAPI. Scale bar, 100 μm. Statistical significance was calculated using the unpaired Student’s t-test (*p < 0.05, **p < 0.01), control = 1. Data are represented as the mean ± SD (n = 3). b Intercellular accumulation of each substrate of efflux transporters. iBMELCs were incubated with Hoechst 33342 (20 μM) or rhodamine 123 (10 μM) in the absence or presence of Ko143 (20 μM) or cyclosporin A (10 μM), respectively for 60 min at 37 °C. Relative fluorescence intensity values were normalized to protein content and self-normalized to the conditions without inhibitor. Data are represented as the mean ± SD (n = 3). Statistical significance was calculated using the unpaired Student’s t-test (***p < 0.001), vehicle of control = 1

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