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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells

Fig. 2

Tight junction analysis. a Effects of A-83-01 on the TEER values of BMECs derived from multiple iPSC lines. Statistical significance was calculated using the unpaired Student’s t-test (***p < 0.001). Data are represented as the mean ± SD (610B1, n = 3; 648A1, 606A1 n = 4). b Effects of TGF-β inhibitors (A-83-01, SB-431542, and RepSox) on the TEER values of BMECs. Statistical significance was calculated using one-way ANOVA and Tukey test (***p < 0.001), vs. control. Data are represented as the mean ± SD (610B1, n = 3). c Lucifer Yellow permeability coefficient (Pe) in iBMELCs. Statistical significance was calculated using the unpaired Student’s t-test (*p < 0.05). Data are represented as the mean ± SD (n = 3). d Immunofluorescence for the tight junction markers (ZO-1, occludin, and claudin-5). Blue: DAPI. White arrows: fragile and discontinuous tight junctions. Scale bar, 50 μm. e Time-course of the TEER values of BMECs derived from multiple iPSC lines. Statistical significance was calculated using two-way repeated measures ANOVA (***p < 0.001). Data are represented as the mean ± SD (648A1, n = 6; 610B1, 606A1, n = 4)

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Fluids and Barriers of the CNS

ISSN: 2045-8118